Analysis of the two populations, Eastern Monarchs and Western Monarchs:
- Differences in flight performance
- Correlation in the genetic profiles to check for distinction in the two populations
2022-05-09
Analysis of the two populations, Eastern Monarchs and Western Monarchs:
Steps followed in the duration of this project-
This is the final joined dataset we used for our analysis-
## # A tibble: 6 × 21 ## ID Gene_379 Gene_203 Gene_C2 Gene_C4 Gene_C5 Gene_C7 Sex Population premass postmass ## <chr> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl> <chr> <chr> <dbl> <dbl> ## 1 FL35 -5.74 -5.53 -4.80 -4.46 -4.42 -4.77 F east 0.470 0.462 ## 2 FL72 -6.21 -6.37 -4.73 -4.46 -4.46 -5.02 F east 0.540 0.524 ## 3 FL74 -4.86 -4.59 -2.65 -2.61 -2.90 -2.84 F east 0.522 0.511 ## 4 FL75 -6.14 -6.31 -4.84 -4.34 -4.44 -4.87 F east 0.564 0.540 ## 5 FL79 -4.40 -4.18 -3.01 -2.77 -3.18 -2.98 F east 0.581 0.580 ## 6 FL05 -6.00 -6.18 -4.94 -4.79 -4.25 -4.71 M east 0.499 0.493 ## # … with 10 more variables: weightloss <dbl>, time.sec <dbl>, time.min <dbl>, distance <dbl>, ## # averagevelocity <dbl>, maxvelocity <dbl>, power <dbl>, Area <dbl>, PC1_wing_size <dbl>, ## # PC2_wing_shape <dbl>
plots = map(numeric_ones,
~datadistribution_plot("Population",
.,
my_data_clean))
Fig.2-Violin plot of average velocity
Fig.3-Violin plot of weight loss
We performed T-test on flight performance variables and observed the following:
Genes 203 and 379 are heavily downregulated in both populations
Genes C2, C4, C5 and C7 seem to be comparatively more downregulated in Eastern Monarchs
Fig.5-Box plots of gene expression
Fig.6-Heat maps of gene expression
Logistic regression to determine significance of gene expression on populations
Performing PCA on the data, we obtained the following results-
Fig.8-PCA on population
Fig.9-PCA on distance class
From our analysis we can conclude the following about the Eastern and Western Monarchs: